Journal: Current Biology

Article Title: Presynaptic and postsynaptic determinants of claustro-cortical connectivity

doi: 10.1016/j.cub.2025.05.056

Figure Lengend Snippet: Figure 3. CLA projections selectively target interneuron populations (A) Illustration depicting experimental paradigm. Briefly, ChR2 was injected using an anterograde viral vector into the CLA (100 nL, 300 nL/min) into different transgenic mouse lines with fluorescent reporters to identify interneuron populations. Paired whole-cell patch-clamp recordings were made in the ACC, and CLA axons were photostimulated to investigate postsynaptic responses. Differential interference contrast (DIC) image of paired recordings of an interneuron and a putative pyramidal neuron. (B) Postsynaptic responses recorded in a layer 5/6 neuron in the presence of gabazine. The postsynaptic response was subsequently removed on bath application of TTX and recovered with TTX and 4AP, confirming that the input was monosynaptic. The subsequent postsynaptic response is abolished with AMPA and NMDA receptor antagonists, NBQX and APV, confirming that the input is excitatory. (C) Illustration of paired recordings of PV interneurons and pyramidal neurons. Confocal image of the ACC shows that PV interneurons are in layers 2/3 and 5/6 of the ACC. A total of 75 PV interneurons (n = 42 in layer 2/3, n = 33 in layer 5/6) were studied. Example trace of a PV interneuron. Representative postsynaptic responses in paired recordings between PV (orange trace) and pyramidal neuron populations (black trace). 69% of all recorded PV neurons were found to be responsive. (D) SOM: confocal image of the ACC shows that SOM interneurons are in layers 2/3 and 5/6 with neurites in layer 1. A total of 48 SOM interneurons (n = 1 in layer 1, n = 14 in layer 2/3, n = 33 in layer 5/6) were studied. Example trace of a SOM interneuron. Representative postsynaptic responses in paired recordings between SOM (purple trace) and pyramidal neuron populations (black trace). 35% of all recorded SOM neurons were found to be responsive.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Biocytin (ε-Biotinoyl-L-Lysine) ThermoFisher Cat. # B1592 Cy5-conjugated streptavidin Jackson ImmunoResearch Laboratories RRID: AB_2337245 DAPI (4′,6-diamidino-2-fenilindol, dihidrocloruro) ThermoFisher Cat. # D1306; RRID AB_2629482 Bacterial and virus strains pAAV2-CaMKIIa-hChR2(H134R)-EYFP Karl Deisseroth Cat. # 26969-AAV2; RRID:Addgene_26969 AAV5.EF1.dflox.hChR2(H134R)-mCherry.WPRE.hGH Karl Deisseroth Cat. # 20297; RRID: Addgene_20297 AAV5.EF1.dflox.hChR2(H134R)-eYFP.WPRE.hGH Karl Deisseroth Cat. # 20298; RRID: Addgene_20298 pAAV-hSyn-DIO-mCherry Addgene Cat. # 50459; RRID:Addgene_50459 Red Retrobeads Lumafluor https://lumafluor.com/ Chemicals, peptides, and recombinant proteins Isoflurane, Forene AbbVie AB (Solna, Sweden) Cat. #: 506949 Sodium pentobarbital APL AB (Stockholm, Sweden) Cat. #: 338327 Temgesic 0.3mg/ml Indivior Europe Limited (Apoteket) Cat. # 521634 Viscotears 2mg/g Thea (Apoteket) Cat. #: 569173 Xylocain Spray 100 mg/ml AstraZeneca Cat. #: 469429 Adhese Universal Refill Bottle 2x5g IvoClar Vivadent Cat. #: 663721WW Dental cement, Tetric EvoFlow, 1x 2 g IvoClar Vivadent Cat. #: 595953WW Silicone sealant Kwik-Cast WPI Cat. #: KWIK-CAST D-APV Tocris Cat. # 79055-68-8 NBQX disodium salt Tocris Cat. # 479347-86-9 Tetrodotoxin citrate (TTX) Tocris Cat. # 18660-81-6 4-Aminopyridine (4AP) Tocris Cat. # 504-24-5 SR-95531 (Gabazine) Merck Cat. # 104104-50-9 DiI (1,1′-dioctadecyl-3,3,3′3’tetramethylindocarbocyanine perchlorate) Invitrogen, ThermoFisher Cat. # D282 Critical commercial assays RNAscope Multiplex Fluorescent v2 ACD Cat. # 323100 Probe- Mm-Slc17a6-C1 ACD Cat. # 319171 Probe- Mm-CamkIIa-C2 ACD Cat. # 445231 TSA Cyanine 3 Akoya Biosciences Cat. #NEL744001KT TSA Cyanine 5 Akoya Biosciences Cat. #NEL745001KT Experimental models: Organisms/strains Mouse, C57BL/6J The Jackson Laboratory RRID: IMSR_JAX: 000664 Mouse, PV-Cre The Jackson Laboratory RRID: IMSR_JAX:017320 Mouse, SOM-Cre The Jackson Laboratory RRID: IMSR_JAX:018973 Mouse, Ai9 (RCL-tdT) The Jackson Laboratory RRID: IMSR_JAX:007909 Mouse, NPY-Cre The Jackson Laboratory RRID: IMSR_JAX:027851 Mouse, 5Ht3a-Cre The Rockefeller University, GENSAT RRID: MMRRC_036680-UCD Mouse, Vglut2-ires-cre The Jackson Laboratory RRID: IMSR_JAX:016963 Software and algorithms ImageJ FIJI NIH https://imagej.nih.gov/ij QuickNII NITRC https://quicknii.readthedocs.io/ VisuAlign NITRC https://visualign.readthedocs.io Python Python Software Foundation https://www.python.org/ (Continued on next page) Current Biology 35, 3174–3190.e1–e5, July 7, 2025 e1

Techniques: Injection, Plasmid Preparation, Transgenic Assay, Patch Clamp

Journal: Cell Reports

Article Title: Restriction factor screening identifies RABGAP1L-mediated disruption of endocytosis as a host antiviral defense

doi: 10.1016/j.celrep.2022.110549

Figure Lengend Snippet:

Article Snippet: Cercopithecus aethiops: Vero-CCL81 cells , ATCC , CCL-81.

Techniques: Virus, Recombinant, Transfection, Protease Inhibitor, Magnetic Beads, Electron Microscopy, Reverse Transcription, Cell Viability Assay, Mutagenesis, Cloning, Luciferase, Staining, Labeling, Software, Real-time Polymerase Chain Reaction, Imaging, Laser-Scanning Microscopy, Microscopy

Journal: eLife

Article Title: Specific Eph receptor-cytoplasmic effector signaling mediated by SAM–SAM domain interactions

doi: 10.7554/eLife.35677

Figure Lengend Snippet: ( A ) Selected images showing the morphologies of DU145 cells expressing the wild-type or various mutants of EphA2 before and after ephrin A1-Fc treated. Cells were stained for F-actin with fluorescent phallodin (red), and nuclei were labelled with DAPI (blue). ( B ) Quantification of the cell areas of DU145 cells expressing various constructs. Data represents the mean ±SEM from four independent experiments with each experiment of at least 400 cells (***, p<0.001 by Two-way ANOVA with multiple comparisons test). ( C ) Western blot analysis showing the expression levels of different EphA2 (anti-Flag) constructs.

Article Snippet: Cell line (Human) , DU145 , ATCC , Cat #HTB-81; RRID: CVCL_0105.

Techniques: Expressing, Staining, Construct, Western Blot

Journal: eLife

Article Title: Specific Eph receptor-cytoplasmic effector signaling mediated by SAM–SAM domain interactions

doi: 10.7554/eLife.35677

Figure Lengend Snippet: ( A ) Selected images showing the morphologies of DU145 cells infected with lenti-virus expressing EphA2 or the GFP control, with or without SHIP2-SAM co-expression. These cells were further divided into two groups, with or without ephrin A1-Fc treatment. SHIP2-SAM was detected by antibody against Myc-tag (green). Cells were stained for F-actin with fluorescent phalloidin (red) and nuclei were labeled with DAPI (blue). ( B ) Quantification of the cell sizes of DU145 cells expressing various proteins. Data represent the mean ± SEM from four independent experiments with each experiment of at least 500 cells (***, p<0.001 by Two-way ANOVA with multiple comparisons test). ( C ) Western blot analysis showing the expression levels of EphA2 (anti-Flag) and SHIP2-SAM (anti-Myc) in transfected cells in each group. GAPDH serves as the loading control.

Article Snippet: Cell line (Human) , DU145 , ATCC , Cat #HTB-81; RRID: CVCL_0105.

Techniques: Infection, Virus, Expressing, Control, Staining, Labeling, Western Blot, Transfection

Journal: eLife

Article Title: Specific Eph receptor-cytoplasmic effector signaling mediated by SAM–SAM domain interactions

doi: 10.7554/eLife.35677

Figure Lengend Snippet:

Article Snippet: Cell line (Human) , DU145 , ATCC , Cat #HTB-81; RRID: CVCL_0105.

Techniques: Transfection, Construct, Plasmid Preparation, Recombinant, Cloning, Software